Minimizing technical variation during sample preparation prior to label-free quantitative mass spectrometry
نویسندگان
چکیده
منابع مشابه
Minimizing technical variation during sample preparation prior to label-free quantitative mass spectrometry.
Sample preparation is the crucial starting point to obtain high-quality mass spectrometry data and can be divided into two main steps in a bottom-up proteomics approach: cell/tissue lysis with or without detergents and a(n) (in-solution) digest comprising denaturation, reduction, alkylation, and digesting of the proteins. Here, some important considerations, among others, are that the reagents ...
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ژورنال
عنوان ژورنال: Analytical Biochemistry
سال: 2015
ISSN: 0003-2697
DOI: 10.1016/j.ab.2015.08.018